Comprehensive information on Nitrogen Use Efficiency for cereal crop production

Effect of Long-Term N fertilization on Soil Organic C and total N in continuous Wheat Under Conventional Tillage in Oklahoma.  Soil and Tillage Res. 47:323-330.

W.R. Raun*, G.V. Johnson, S.B. Phillips and R.L. Westerman

 044 North Ag. Hall, Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 74078. Contribution from the Okla. Agric. Exp. Stn.  * - Corresponding author. TEL: (405) 744-6414. FAX: (405) 744-5269.

 

Abstract

Fertilizer nitrogen (N) can impact on soil total N and organic C.  The effects of long-term nitrogen (N) applications in continuous winter wheat (Triticum aestivum L.) production systems on total N and organic C in soils has not previously been studied.  Deep soil cores were taken from four long-term winter continuous wheat experiments to evaluate differences in total N and organic carbon (C) as affected by more than 23 years of annual N applications.  When N was applied at rates 90 kg ha-1, surface soil (0-30 cm) organic C was either equal to that of the check (no N applied) or slightly greater.  Total soil N (0-30 cm) increased at the high N rates at all locations.  However, at two locations, total soil N decreased at low N rates, indicating the presence of priming (increased net mineralization of organic N pools when low rates of fertilizer N are applied).  At these same two sites, soil-plant inorganic N buffering (amount of N that could be applied in excess of that needed for maximum yield without resulting in increased soil profile inorganic N accumulation) was greater compared to the other two sites where no evidence of priming was found.  In general, C:N ratios increased at the low rates of applied N and then decreased to levels below that found in check plots at high N rates (134 kg N ha-1 yr-1).  Combined surface (0-30 cm) soil analyses of total N and organic C were useful in detecting where priming had taken place and where soil-plant inorganic N buffering was expected to be high in these long-term N fertilization experiments.  Predictability of the priming effect combined with soil-plant inorganic N buffering should assist us in establishing environmentally safe N rates.  Surface soil (0-30 cm) organic C increased when N was applied at rates in excess of that required for maximum yield.

 

Keywords: Organic carbon, total nitrogen, wheat, priming, buffering

 

Introduction

            Analyses of total nitrogen (N) and organic C in soils has been reported in numerous articles dealing with continuous cropping production systems.  However, few have assessed changes in total N and organic C in soils over time.  In addition, the resultant effect of annual applications of N in continuous wheat production systems on total soil N and organic C has not been monitored. 

            Blevins et al. (1983) found a 37 and 12% increase in soil organic C under NT and CT corn, respectively after 10 yr of applying 84 kg N ha-1 yr-1 at the 0-5 cm depth.  In continuous corn experiments conducted by Havlin et al. (1990), high N rates (252 kg N ha-1) were found to increase surface soil (0-30 cm) organic C levels.

            MacVicar et al. (1951) found that the lowest 15N recoveries were associated with a low level of soil organic matter and a high level of nitrogen addition.  Most of the soil nitrogen was maintained in a reduced state as bacterial cellular nitrogen in soils with a high C:N ratio (MacVicar et al., 1951).  When the soil C:N ratio was small, free nitrogen including nitrate accumulated and denitrification was assumed to occur (MacVicar et al., 1951). 

            Varvel and Peterson (1990a) indicated that the differences in estimating fertilizer N recovery using isotopic and difference methods were due to synchronization problems between N mineralization and crop N use.  This was probably due to cropping system, previous crop, amount and type of residue and other environmental factors.  However, Varvel and Peterson (1990a) did indicate that either method is satisfactory within a specific cropping system, but that neither method does well across diverse cropping systems where differences in immobilization could occur.  Nitrogen removal in the grain accounted for 50% of the applied N in continuous grain sorghum and corn systems at low N rates of 34 and 90 kg N ha-1, respectively (Varvel and Peterson, 1990b).  At higher N rates of 68 and 180 kg N ha-1 for grain sorghum and corn systems, only 20 to 30% of the applied N was accounted for by N removal in the grain.  This difference in percent fertilizer N removed in the grain was noted to be a function of immobilization by crop residues and soil organic matter and not due to N leaching (Varvel and Peterson, 1990b). This was supported by observations made for NH4-N and NO3-N concentrations from soil profile analysis (0-150 cm), whereby no differences were observed from samples taken four years apart.

            Olson (1982) found that 81.9% of the fertilizer N remaining in a 0-150 cm soil profile had been immobilized by harvest time in a winter wheat experiment.  In addition, 70% of the fertilizer N remained in the 0-10 cm profile of a silt loam soil at all sampling dates (October 1979 through June, 1980).  Immobilization of the fertilizer N in the 0 to 10 cm layer limited downward movement, plant uptake and losses.  Most of the N not immobilized was used by the crop when N was applied at a rate of 80 kg ha-1.  At maturity, only 18% of the fertilizer N in the 150-cm profile was present as inorganic N.  Groot and de Willigen (1991) suggested that N can be immobilized almost immediately after application, without increased metabolic activity of the microbial biomass.

            The objective of this study was to evaluate the effects of long-term N applications in continuous winter wheat on total soil N and organic C in surface horizons.

 

 

 

Materials and Methods

            Four long-term (>23 years) continuous winter wheat fertility experiments were sampled in 1993 to determine total N, organic C, NH4+-N and NO3--N within the soil profile.  The four experiments are identified as 222, 406, 502 and 505.  Experiments 502 and 505 were separate studies conducted at the same location.  Soil types were Kirkland silt loam (fine-mixed thermic Udertic Paleustoll), Tillman clay loam (fine, mixed, thermic Typic Paleustoll) and Grant silt loam (fine‑silty, mixed, thermic Udic Argiustoll) for experiments 222, 406, and 502 and 505, respectively.  Additional site information is provided in Table 1.  Fertilizer treatments and surface (0-30 cm) soil test analyses at the time all trials were sampled in 1993 are reported by location in Table 2.  Fertilizer treatments reported in Table 2 were applied preplant in the fall of each year and incorporated prior to planting.  Winter wheat was planted in 25.4 cm rows at seeding rates of 67 kg ha-1 at all locations.  All sites were managed under conventional tillage (disk incorporation of wheat straw residues following harvest and prior to planting) with a maximum tillage depth ranging from 15 to 25 cm. 

            Three soil cores 4.45 cm in diameter, were taken from each plot to a depth of 240 cm and sectioned in increments of 0 to 15, 15 to 30, 30 to 45, 45 to 60, 60 to 90, 90 to 120, 120 to 150, 150 to 180, 180 to 210 and 210 to 240 cm.  Soil samples were air dried at ambient temperature and ground to pass a 100-mesh sieve (<0.15 mm) for total N and organic C analyses (Tabatabai and Bremner, 1970).  Soils were analyzed for total nitrogen and organic C (non-calcareous soil) using a Carlo-Erba (Milan, Italy) NA 1500 dry combustion analyzer (Schepers et al., 1989).  For experiment 406, soil organic C was determined by digestion with an acidified dichromate (K2Cr2O7-H2SO4) solution (Yeomans and Bremner, 1988) due to the presence of free CaCO3 in surface horizons. Duplicate soil samples were also extracted using 2M KCl (Bremner, 1965) and analyzed for NH4‑N and NO3‑N using an automated flow injection analysis system.  Soil pH was determined using a glass electrode and a soil/water ratio of 1:1.

            The center 3.05 m of each plot was harvested for grain yield using a conventional self-propelled combine, and wheat straw was uniformly redistributed in all plots each year.

Fertilizer N recovery in the grain was determined by multiplying treatment grain yield x grain N, subtracting check (no N fertilization) grain yield x grain N and dividing by the rate of N applied.

 

Results

Previous Studies

            This manuscript is an extension of work on the same long-term winter wheat experiments reported by Westerman et al. (1994), Raun and Johnson (1995) and Johnson and Raun (1995).  Initial work by Westerman et al. (1994) documented accumulation of NH4-N and NO3-N in the soil profiles following long term annually applied fertilizer N rates in winter wheat.  This work concluded that no accumulation of NH4-N and NO3-N occurred in soil profiles at recommended N rates where maximum yields were obtained.  Raun and Johnson (1995) and Johnson and Raun (1995) proposed a soil-plant buffering concept to explain why soil profile inorganic N did not begin to increase until N rates in excess of that required for maximum yield were applied.  Loss of N from the soil-plant system can take place via plant N volatilization, denitrification and surface volatilization when N rates exceed that required for maximum yield.  Also, increased grain N, straw N, organic N and C in the soil are found when N rates exceed that needed for maximum yields.  The soil-plant buffering concept helped to explain why unaccounted N should not be immediately attributed to leaching in studies where these biological mechanisms remained active.  Grain yield optimums over the 23+ year period included in each of these experiments were found at 56, 47, 62 and 43 kg N ha-1yr-1 for experiments 222, 406, 502 and 505, respectively (Raun and Johnson, 1995).  Significant increases in soil profile inorganic N were not reported until N was applied at rates of 104, 75, 85 and 99 kg ha-1yr-1 at these same respective locations (Raun and Johnson, 1995).  The difference between the observed N rate where soil profile inorganic N accumulation became significant and the N rate where maximum yields were obtained is an estimate of the soil-plant buffering capacity or the ability of the soil-plant system to limit inorganic N accumulation when N rates exceed that required for maximum yield.   Therefore, on an annual basis, 48, 28, 23 and 55 kg of N fertilizer ha-1 could have been applied in excess of requirements for maximum yield in experiments 222, 406, 502 and 505 without increasing inorganic N accumulation or the risk of NO3-N leaching.

            The expression of treatment on total soil N and organic C was expected to be different in the surface soil profile (0-15 cm) compared to soil at other depths, largely due to annual disking to a depth of 15 cm, which led to mechanical mixing and aeration.  The extent of root proliferation was expected to be greatest within the top 0-30 cm.  The combined effects of increased microbial activity, root proliferation and cultivation in the surface 0-30 cm led us to select this depth for more detailed analyses.  Results were consistent with this approach, since few differences in total soil N or organic C were noted at depths > 60 cm (data not reported). 

 

Total soil N

            Nitrogen fertilization significantly increased (linear and/or quadratic) total soil N in the surface 30 cm at all locations (Table 3 and Figure 1).  This was most apparent when the high rate was compared to the check (no N fertilization).  Although this significant linear increase (over all rates) was found, total soil N tended to decrease at the low N rates in experiments 222 and 505.  However, when N was applied at rates in excess of that needed for maximum yield, total soil N increased in all experiments. 

 

Organic C

            Similar to observations for total soil N, soil organic C increased with increasing N applied at three of the four sites (Table 3 and Figure 2).  This was consistent with work by Blevins et al. (1983) and McAndrew and Malhi (1992) who demonstrated increases in soil organic C with increasing N applied.  At experiments 222 and 505, soil organic C did not increase until at least 67 kg N ha-1yr-1 was applied (Table 3 and Figure 2).  A tendency for increased soil organic C when N was applied at rates in excess of that required for maximum yield was noted at all locations.

 

Carbon Nitrogen Ratio

            In experiments 222, 502 and 505, applied N significantly affected C:N ratios (Table 3 and Figure 3).  In these experiments, C:N ratios increased at the low to moderate N rates but then decreased to levels below that observed in the check in experiments 222 and 502. (Figure 3). We hypothesize that, similar to the work of Westerman and Kurtz (1973), applied N at rates <= 67 kg ha-1 was expected to have a 'priming effect' resulting in increased net mineralization of N from the soil organic matter pool.  This was evident in the higher C:N ratios at the low N rates, largely due to decreased total soil N since organic C levels were in general unaffected within this same range (annual N <= 67 kg N ha-1).  Several authors have found that N rates which exceed that required for maximum yields generally result in decreased harvest indices and associated higher straw yields in wheat.  This would aid in explaining why organic C levels increased at the higher N rates used in these trials.  However, in order for total soil N levels to be significantly lower at the low to moderate N rates, applied N was expected to have a different effect on the organic N pool.  Westerman and Kurtz (1973) suggested that increased crop soil N uptake was due to stimulation of microbial activity by N fertilizers which increased mineralization of soil N, thus making more soil N available for plants.  Similarly, what could be a 'priming effect' in these experiments occurred at the low to moderate applications of fertilizer N. 

 

Fertilizer recovery in the grain

            Estimates of fertilizer N recovery in the grain using the difference method are reported in Table 4.  At the low N rates, 30-60% of the N applied could be accounted for in the grain.  Annual N rates in excess of 90 kg N ha-1 resulted in fertilizer N recovery in the grain of less than 28% at all locations.  Varvel and Peterson (1990a) have indicated that problems of estimating N recovery in crop production using the difference method include the assumption that mineralization, immobilization and other N transformations are the same for both fertilized and unfertilized soils.

           

Discussion

            At experiments 222 and 505 a significant decrease in surface soil total N was noted when N was applied at annual rates between 45 and 90 kg ha-1.  Therefore, continued microbial breakdown of soil organic matter may have caused the decrease in total soil N with no corresponding change in organic C since increased growth and straw biomass (via priming) would have been present.  At the higher N rates which exceeded that required for maximum yields (>90 kg N ha-1), organic C levels were equal to or somewhat greater than the check.  We think that evidence of priming (increased net mineralization of organic N pools when low rates of fertilizer N are applied) observed here took place within the first five years in these long-term studies.

            It was interesting to find that estimates of soil-plant inorganic N buffering (rate of N that can be applied in excess of that needed for maximum yield without resulting in increased soil profile inorganic N accumulation) were greatest in experiments 222 and 505 (Raun and Johnson, 1995) where evidence of the priming effect was also observed.  As indicated earlier, we hypothesize that priming took placed since decreased total soil N at low rates in two of these long-term experiments was observed.  Consistent with this, it is thought that soil-plant buffering will be greater in soils where priming is observed, a result of increased N from easily mineralizable N pools.  Therefore, these soil-plant environments are also capable of immobilizing excess mineral N.  However, it should be mentioned that differences in total soil N that were due to treatment reported here would not likely be detected in short-term (3-5 year) studies given the precision at which total N can be determined using dry combustion methods (0.01% or 0.10 g kg-1)

            The combined use of total N and organic C in relation to N applied in these long-term trials provided reasonable evidence of the priming effect proposed by Westerman and Kurtz (1973).  Analyses for surface soil organic C alone was useful in detecting increases at the high N rates (site specific) but provided little information when compared across locations (no relationship with soil-plant inorganic N buffering or total soil N).  The combined use of total organic C and lignin (highly stable) content may be a more useful tool since the easily mineralizable N fractions will depend on organic C stability.  This work further suggests that the quantity of easily mineralizable N should be a reliable predictor of soil-plant inorganic N buffering since total N decreased at low N rates (easily mineralized N), at both locations where soil-plant inorganic N buffering was large.  If easily mineralizable N could be determined on a routine basis, it may provide an index for determining environmentally safe N rates for winter wheat production.  

 

 


 

References

Blevins, R.L., G.W. Thomas, M.S. Smith, W.W. Frye and P.L. Cornelius. 1983. Changes in soil properties after 10 years of continuous non-tilled and conventionally-tilled corn. Soil Tillage Res. 3:135-136.

Bremner, J.M. 1965. Inorganic forms of nitrogen. In C.A. Black et al. (ed.) Methods of soil analysis, Part 2. Agronomy 9:1179-1237. Am. Soc. of Agron., Inc., Madison WI.

Groot, J.J.R., and P. de Willigen. 1991. Simulation of the nitrogen balance in the soil and a winter wheat crop. Fertilizer Research 27:261-272.

Havlin, J.L., D.E. Kissel, L.D. Maddux, M.M. Claassen and J.H. Long. 1990. Crop rotation and tillage effects on soil organic carbon and nitrogen. Soil Sci. Soc. Am. J. 54:448-452.

Johnson, G.V., and W.R. Raun. 1995. Nitrate leaching in continuous winter wheat: use of a soil-plant buffering concept to account for fertilizer nitrogen. J. Prod. Agric. 8:486-491.

MacVicar, R., W.L. Garman and R. Wall. 1951. Studies on nitrogen fertilizer utilization using 15N.  Soil Sci. Soc. Am. Proc. 15:265-268.

McAndrew, D.W., and S.S. Malhi. 1992. Long-term N fertilization of a solonetzic soil: effects on chemical and biological properties. Soil Biol. Biochem. 24:619-623.

Olson, R.V. 1982. Immobilization, nitrification, and losses of fall-applied, labeled ammonium-nitrogen during growth of winter wheat. Agron. J. 74:991-995.

Raun, W.R., and G.V. Johnson. 1995. Soil-plant buffering of inorganic nitrogen in continuous winter wheat.  Agron. J. 87:827-834.

Schepers, J.S., D.D. Francis, and M.T. Thompson, 1989. Simultaneous determination of Total C, Total N, and 15N on Soil and Plant Material. Commun. In Soil Sci. Plant Anal. 20,949-959

Tabatabai, M.A and J.M. Bremner, 1970. Use of the Leco Automatic 70-Second Carbon Analyzer for total Carbon Analysis of soils. Soil Sci. Soc. Amer. Proc. 34,608-610.

Varvel, G.E., and Todd Andrews Peterson. 1990a. Nitrogen fertilizer recovery by corn in monoculture and rotation systems. Agron. J. 82:935-938.

Varvel, G.E., and Todd Andrews Peterson. 1990b. Residual soil nitrogen as affected by continuous, two-year, and four-year crop rotation systems. Agron. J. 82:958-962.

Westerman, R.L., and L.T. Kurtz. 1973. Priming effect of 15N-labeled fertilizers on soil nitrogen in field experiments. Soil Sci. Soc. Amer. Proc. 37:725-727.

Westerman, R.L, R.K. Boman, W.R. Raun and G.V. Johnson. 1994. Ammonium and nitrate nitrogen in soil profiles of long-term winter wheat fertilization experiments. Agron. J. 86:94-99.

Yeomans, J.C., and J.M. Bremner. 1988. A rapid and precise method for routine determination of organic carbon in soil. Commun. Soil Sci. Plant Anal. 19:1467-1476.


 

List of Figures

 

Figure 1.  Surface (0-30 cm) soil total N as affected by annual applications of fertilizer N, experiments 222, 406, 502 and 505 (SED -standard error of the difference between two equally replicated means).

 

Figure 2.  Surface (0-30 cm) soil organic C as affected by annual applications of fertilizer N, experiments 222, 406, 502 and 505 (SED -standard error of the difference between two equally replicated means).

 

Figure 3.  Surface (0-30 cm) soil organic C:total N ratios as affected by annual applications of fertilizer N, experiments 222, 406, 502 and 505 (SED -standard error of the difference between two equally replicated means).


 

Table 1.  Long-term experiment included in the analysis, year established, soil core sampling date, crop years prior to sampling, annual average rainfall and  range in annual rainfall (all sites were continuous winter wheat under conventional tillage).

___________________________________________________________________________________________

Exp.      Long., Lat.       Year              Number of     Dates           Crop Years Annual avg.                       Mean Annual

                                        Est.               Replications Sampled     Prior to         Rainfall      Range          Temperature,

                                                                                                            Sampling                                               

___________________________________________________________________________________________

                                                                                                                                   ----------- mm -----------    C

 222      36 7' 7"N        1969             4                      July 1993     24                 922               606-1493    15.0

              97 5' 30" W

 406      34 36' 34" N  1965             4                      July 1993     28                 670               295-1141    17.1

              99 20' 0" W

 502      36 23' 13" N  1970             4                      July 1993     23                 771               503-1314    15.6

              98 6' 29" W

 505      36 23' 13" N  1970             3                      July 1993     23                 771               503-1314    15.6

              98 6' 29" W

___________________________________________________________________________________________

- obtained from the years each study was conducted up until soil profile sampling and analyses was performed.

 


 

Table 2.  Surface soil test characteristics (0-30 cm) in 1993 for experiments 222, 406, 502 and 505.

__________________________________________________________________________

Experiment         Fertilizer Applied                                       Soil Test Level           

                          N               P             K                       pH                       P                     K                      

__________________________________________________________________________

                          --------- kg ha-1 yr-1---------                                            mg kg-1          mg kg-1           

222                   0                29           38                     5.85                    51                   218                  

                          45             29           38                     5.84                    38                   200                  

                          90             29           38                     5.80                    34                   155                  

                          134           29           38                     5.73                    26                   130                  

   SED                                                                           0.08                    11                   36

406                   0                0              0                        7.29                    9                      409

                          45             20           38                     7.13                    32                   445

                          90             20           38                     7.38                    25                   432

                          134           20           38                     7.12                    24                   445

                          179           20           38                     6.79                    23                   442

   SED                                                                           0.18                    3                      14                                                                               

502                   0                20           56                     5.95                    70                   488

                          22             20           56                     5.83                    66                   438

                          45             20           56                     5.76                    71                   467

                          67             20           56                     5.67                    75                   455

                          90             20           56                     5.60                    72                   468

                          112           20           56                     5.49                    83                   457                  

   SED                                                                           0.14                    17                   38

505                   0                29           56                     5.74                    119                 420

                          34             29           56                     5.43                    94                   343

                          67             29           56                     5.24                    103                 340

                          134           29           56                     5.04                    121                 413

                          269           29           56                     4.85                    93                   366

   SED                                                                           0.10                    14                   28                                                    

_______________________________________________________________________________

pH,  1:1 soil:water;  K and P, Mehlich III

SED - standard error of the difference between two equally replicated means.


 

Table 3.  Analysis of variance, mean squares and associated contrasts for total soil N, organic C and Carbon:Nitrogen ratios, experiments 222, 406, 502 and 505 for the 0-30 cm soil depth, 1993.

___________________________________________________________________________________

Source of Variation                     df                      Total N                    Organic C            C:N

___________________________________________________________________________________

                                                                                ------------------------ Mean Squares -------------------------

                                                                                                                 #222                                                 

Rep                                                3                       0.0087                    1.176                    1.109**

Trt                                                   3                       0.0133                    0.217                    0.526

Error                                              25                    0.0065                    0.537                    0.193

Single degree of freedom contrasts

N Rate linear                               1                       0.0074                    0.374                    0.006

N Rate quadratic                         1                       0.0314*                  0.220                    1.568*

0 vs 134                                        1                       0.0085                    0.431                    0.009 

                                                                                                                 #406                                                 

Rep                                                3                       0.0112                    2.903*                   10.162*                                             

Trt                                                   4                       0.0055                    1.320                    1.871

Error                                              32                    0.0068                    0.986                    2.759

Single degree of freedom contrasts

N Rate linear                               1                       0.0195**                 4.408*                   1.175

N Rate quadratic                         1                       0.0005                    0.012                    0.734

0 vs 134                                        1                       0.0036                    2.182@                2.992

                                                                                                                 #502                                                 

Rep                                                2                       0.0003                    0.592*                   1.435**              

Trt                                                   5                       0.0094**                 0.332                    0.497**

Error                                              28                    0.0013                    0.154                    0.123

Single degree of freedom contrasts

N Rate linear                               1                       0.0317**                 0.800*                   0.371@

N Rate quadratic                         1                       0.0025                    0.844*                   0.512*

0 vs 112                                        1                       0.0220**                 0.527@                0.262

                                                                                                                 #505                                                 

Rep                                                2                       0.03604*                1.175                    2.262

Trt                                                   4                       0.00763                  2.694                    1.014

Error                                              23                    0.00644                  1.750                    3.399

Single degree of freedom contrasts

N Rate linear                               1                       0.01903@              5.549@                0.832

N Rate quadratic                         1                       0.00241                  0.002                    0.626

0 vs 134                                        1                       0.01153                  7.000*                   3.316

___________________________________________________________________________________

**,*,@ - significant at the 0.01, 0.05 and 0.10 probability levels, respectively.


 

Table 4.  Total soil profile inorganic N, average annual grain yield, total N removed in the grain and estimates of fertilizer N recovery, experiments 222, 406, 502 and 505.

_______________________________________________________________________________________________

Treatment                             Total soil profile Total N             Average               Total N               Fertilizer N       Fertilizer                                             

                                                inorganic N         Applied            annual grain       removed in        removed in      N recovery,                                                                                                                       yield                  grain                     grain                   grain, %          

                                                  ----------------------------------------------kg ha-1--------------------------------------------------        

_______________________________________________________________________________________________

#222 (0-240 cm) 24 years

0-29-38                                    424                     0                        1329                     692                     0                        0   

45-29-38                                 413                     1080                 1751                     1046                   354                   0.33                                                    

90-29-38                                 432                     2160                 1882                     1156                   464                   0.21                                                    

134-29-38                               608                     3216                 1933                     1401                   708                   0.22                                                    

#406 (0-210 cm) 28 years

0-0-0                                        503                     0                        1416                     900                     0                        0   

45-20-38                                 487                     1260                 1972                     1363                   463                   0.37                                                    

90-20-38                                 509                     2520                 2095                     1589                   689                   0.27                                                    

134-20-38                               622                     3752                 1899                     1483                   583                   0.16                                                    

179-20-38                               745                     5012                 1907                     1447                   547                   0.11                                                    

#502 (0-240 cm) 23 years

0-20-56                                    314                     0                        1727                     844                     0                        0   

22-20-56                                 294                     506                   2240                     1133                   289                   0.57                                                    

45-20-56                                 322                     1035                 2381                     1221                   377                   0.36                                                    

67-20-56                                 310                     1541                 2668                     1399                   555                   0.36                                                    

90-20-56                                 344                     2070                 2749                     1460                   616                   0.30                                                    

112-20-56                               502                     2576                 2655                     1435                   590                   0.23                                                    

#505 (0-300 cm) 23 years

0-29-56                                    384                     0                        1615                     809                     0                        0   

33.6-29-56                              387                     772                   2406                     1261                   451                   0.58                                                    

67.3-29-56                              375                     1545                 2645                     1460                   650                   0.42                                                    

134.5-29-56                            517                     3091                 2721                     1677                   867                   0.28                                                    

269-29-56                               1023                   6182                 2541                     1624                   814                   0.13                                                    

_______________________________________________________________________________________________

In general analyses for total soil N is of little use in short-term experiments since the precision using dry combustion is 0.01% N.   With a bulk density of 1.47 g cm-3 this precision computes to be 241 kg N ha-1 for a 0-15 cm profile.   In experiments where N is applied at rates ranging from 0 to 300 kg N ha-1 each year for 3 to 5 years, it is unlikely that treatment differences will be detected when measuring total soil N.  However, following more than 23 years, total soil N was significantly affected by treatment (Table 3).